dpnii buffer (New England Biolabs)
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New England Biolabs
dpnii buffer
Dpnii Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dpn+ii+buffer/Dpn+II/bio_rxiv__64898__2026__05__06__722996-296-6-17
Average 99 stars, based on 1734 article reviews
Dpnii Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dpn+ii+buffer/Dpn+II/bio_rxiv__64898__2026__05__06__722996-296-6-17
Average 99 stars, based on 1734 article reviews
dpnii buffer - by Bioz Stars,
2026-11
99/100 stars
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Incubation:Article Title: CTCF N-terminal Domain Regulates Clustered Protocadherin Gene Expression by Enhancing Cohesin Processivity Article Snippet: .. Cells were then resuspended in 73 μl of nuclease-free water, followed by the addition of 10 μl of 10 × Article Title: WAVE2 and REST/NRSF Regulate Clustered Gene Expression by Maintaining Heterochromatin Organization Article Snippet: .. Nuclei were pelleted (800 × g, 5 min, 4°C) and resuspended in 73 μl of nuclease-free water, 10 μl of Article Title: CCCTC-binding factor N-terminal domain regulates clustered protocadherin gene expression by enhancing cohesin processivity Article Snippet: .. Cells were then resuspended in 73 μl of nuclease-free water, followed by the addition of 10 μl of 10 × other:Article Title: Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation Article Snippet: ChIP experiments in PIPER-15 cells and with anti-E2A and anti-MED1 antibodies in primary pro- and pre-B cells, were performed according to Nowak et al. ( ) by first cross-linking with 2 mM Disuccinimidyl Glutarate (DSG, Sigma 80424) and then with 1% formaldehyde. Article Title: Cohesin‐mediated DNA loop extrusion resolves sister chromatids in G2 phase Article Snippet: After the final wash, wild-type medium containing 200 ng ml 1 nocodazole (Sigma Aldrich, M1404) was added to the cells. Article Title: Topological screen identifies hundreds of Cp190- and CTCF-dependent Drosophila chromatin insulator elements Article Snippet: Briefly, 2 × 10 7 cells were cross-linked by incubating in fix buffer [2% formaldehyde, 15 mM Hepes (pH 7.6), 60 mM KCl, 15 mM NaCl, 4 mM MgCl 2 , 0.1% Triton X-100, 0.5 mM dithiothreitol, and protease inhibitor cocktail (Roche)] for a total of 10 min at 25°C and 750 rpm on a shaker. Blocking Assay:Article Title: Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase Article Snippet: All centrifugations were performed at 2500 g, apart from those in the ethanol precipitation step, which were performed at 21000 g. Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma), 0.2% Nonidet P-40 substitute (Sigma), 1 × complete EDTA-free protease inhibitor (Roche) for 30 min at 4 °C. .. The DNA was then digested using Dpn II (375 U DpnII (NEB, R0543 T/M/L) in 1× |